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KMID : 0359320020420010073
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Korean Journal of Veterinary Research
2002 Volume.42 No. 1 p.73 ~ p.80
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Comparison of serological methods for detection of avian influenza virus antibodies
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한명국/Han, Myun-guk
박경윤/권용국/김재홍/Park, Kyoung-yoon/Kwon, Yong-kuk/Kim, Jae-hong
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Abstract
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An enzyme-linked immunosorbent assay (ELISA) using purified hemagglutinin of swine influenza virus (H1N1) as antigen was developed for detection of antibody to avian influenza virus (AIV). The sensitivity and specificity of a developed and commercial available ELISA kits were compared with those of agar gel precipitation (AGP) test and hemagglutination inhibition (HI) test using sera collected from chickens under condition of field exposure. The concentration of antigen, serum dilution and concentration of enzyme-conjugated secondary antibody in developed ELISA (S-ELISA) were 0.5㎍/100㎕, 1:200 and 0.03㎍/100㎕, respectively. The correlation coefficients between S-ELISA and commercial ELISA and HI titers were 0.419 and 0.533, respectively. A significant correlation (p ＜ 0.01) was not found between HI and ELISA titers. The S-ELISA was found to be as more sensitive and specific than the AGP test, showing 86.8% sensitivity and 85.3% specificity. It is suggested that the ELISA using the SIV as antigen may be useful method as an investigating tool for AIV serological surveillance.
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